Bacterial Defence
Ana Rita Costa, Daan F. van den Berg, Jelger Q. Esser, Aswin Muralidharan, Halewijn van den Bossche, Boris Estrada Bonilla, Baltus A. van der Steen, Anna C. Haagsma, Ad Fluit, Franklin L. Nobrega, Pieter-Jan Haas, Stan J.J. Brouns
Clinical Pseudomonas aeruginosa strains accumulate defense systems to obtain panphage resistance.
Gene Delivery
Aswin Muralidharan, Pouyan E. Boukany
We reviewed the latest trends in electrotransfer of nucleic acids and proteins.
Rūta Palepšienė, Aswin Muralidharan, Martynas Maciulevičius, Paulius Ruzgys, Sonam Chopra, Pouyan Boukany, Saulius Šatkauskas
We performed a fundamental study of mechanism of co-electrotransfer of oligonucleotides and siRNA simultaneously with plasmid DNA.
Reece W. Lewis, Aswin Muralidharan, Benjamin Klemm, Pouyan E. Boukany, Rienk Eelkema
We developed charge-reversing polycations which can encapsulate DNA and release it at nucleophilic environments. We further demonstrate evidences that these polycations could potentially be used for gene transfection applications.
Maria Scuderi, Janja Dermol-Černe, Clarissa Amaral da Silva, Aswin Muralidharan, Pouyan E. Boukany, Lea Rems
After a critical evaluation, we discovered that existing models are insufficient to simulate the transmembrane transport associated with electroporation and hence must be revisited.
Aswin Muralidharan, Georg R. Pesch, Hendrik Hubbe, Lea Rems, Mahdiyeh Nouri-Goushki, Pouyan E. Boukany
We developed microtrap electroporation, and it works better than conventional bulk electroporation to deliver exogenous biomolecules.
Aswin Muralidharan, Hans Uitenbroek, Pouyan E. Boukany
We tracked electrotransferred DNA cargo transport inside mammalian cells and discovered that it is not the DNA size, but what it encounters in the cytosol that governs its intracellular transport.
Aswin Muralidharan, Lea Rems, Michiel T. Kreutzer, Pouyan Boukany
We revealed that actin networks increase the energy barrier of electroporation, challenging the traditional view that only lipid bilayer membranes are involved in maintaining cell membrane electropermeability.
Shaurya Sachdev, Aswin Muralidharan, Dipendra K. Choudhary, Dayinta L. Perrier, Lea Rems, Michiel T. Kreutzer, Pouyan Boukany
We showed that electrotransfer of DNA to giant unilamellar vesicles is independent of DNA size.
Dayinta L. Perrier, Afshin Vahid, Vaishnavi Kathavi, Lotte Stam, Lea Rems, Yuval Mulla, Aswin Muralidharan, Gijsje H Koenderink, Michiel T. Kreutzer, Pouyan Boukany
We showed that actin networks inside vesicles increase the resealing time after electroporation. Furthermore, the actin networks depolymerize during electroporation.
Microfluidics
Qian Liu, Aswin Muralidharan, Abtin Saateh, Zhaoying Ding, Peter Ten Dijke, Pouyan E. Boukany
We created multi-functional hydrogel based microcarriers inside which we grew three dimensional cancer cell cultures and observed them over several days.
Shaurya Sachdev, Aswin Muralidharan, Pouyan E. Boukany
We use microfluidics to observe the molecular processes involved in breakup of polymeric droplets.
Cardiovascular Flows
Saeed S. Khalafvand, Jason D. Voorneveld, Aswin Muralidharan, Frank J.H. Gijsen, Johan G. Bosch, Theo van Walsum, Alexander Haak, Nico de Jong, Saša Kenjereš
We studied the flow patterns in a left ventricle model using computational fluid dynamics and statistical shape modelling and compared it with particle image velocimetry experiments.
Jason D. Voorneveld, Aswin Muralidharan, Timothy Hope, Hendrik J. Vos, Pieter Kruizinga, Antonius F.W. van der Steen, Frank J.H. Gijsen, Saša Kenjereš, Nico de Jong, Johan G. Bosch
We validated high frame ultrasound particle image velocimetry using gold standard optical particle image velocimetry.